Product Launch (Blog)

Jun, 06 2024

Exploring Innovators in the ELISpot and FluoroSpot Assay Market: An Overview

ELISpot (Enzyme-Linked ImmunoSpot) and FluoroSpot assays are immunological techniques used to detect and quantify secreted cytokines or other proteins at the single-cell level. ELISpot utilizes enzyme-linked antibodies and colorimetric detection, while FluoroSpot employs fluorescently labeled antibodies for multiplex detection, allowing simultaneous measurement of multiple cytokines. A major application of these assays is in vaccine development and immunotherapy research, where they are crucial for evaluating immune responses. By measuring the frequency and functionality of cytokine-secreting cells, such as T cells, these assays help assess the efficacy of vaccines and immunotherapies, enhancing the understanding and optimization of immune responses for disease prevention and treatment.

The Global ELISpot and FluoroSpot Assay Market valued at USD 301.25 million in 2023 is projected to reach 784.00 million by 2031 and will grow at a CAGR of 12.17% during the forecast period of 2024-2031.

Below are the Top ELISpot and FluoroSpot Assay companies with a Significant Market Share:




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BD is a global medical technology company that provides a wide range of products and services for diagnostics, medical devices, and biosciences. In the ELISpot and FluoroSpot assay market, BD offers advanced instrumentation and reagents designed to enhance the sensitivity and accuracy of these assays, facilitating reliable detection and quantification of immune responses, which are critical for research in immunology and vaccine development.

  • Human IFN-g ELISPOT Kit
  • Mouse IFN-g ELISPOT Kit
  • BD ELISPOT Human IL-12 p70 ELISPOT Set
  • Mouse TNF ELISPOT Pair

Europe, Middle East and Africa, South America, North America, and Asia-Pacific

In April 2021, BD announced that the company will be presenting in virtual investor healthcare conferences “The Bank of America Securities 2021 Virtual Healthcare Conference” and “The UBS Global Healthcare Virtual Conference”. This helped the company gain more customers globally and improved revenue for future markets.


Oxford Immunotec USA, Inc.

Oxford Immunotec specializes in innovative immunology-based diagnostic tests, with a strong focus on tuberculosis and other infectious diseases. The company is known for its T-SPOT technology, a type of ELISpot assay that is highly regarded for its sensitivity and specificity in detecting T cell responses. Oxford Immunotec's assays are widely used in clinical diagnostics and research settings to monitor immune function.

  • T-Cell Select
  • T-Cell Xtend

Europe, Middle East and Africa, North America, and Asia-Pacific

In May 2021, Oxford Immunotec USA, Inc. had declared the implementation of T-SPOT Discovery SARS-CoV-2 kit. The test kit was used for T-cell testing in the UK COVID-19 Human Challenge Study. The first stage of the trial commenced in March. The launch would provide a new platform for the ELISPOT methodology and portfolio, and it would establish an optimized dose and study design that will subsequently be used to evaluate the efficacy of treatment and vaccine candidates.



Bio-Techne is a leading biotechnology company that provides high-quality reagents, assays, and instruments for research and clinical diagnostics. In the ELISpot and FluoroSpot assay market, Bio-Techne offers comprehensive solutions, including assay kits and reader systems, which are designed to accurately measure cytokine production and cell-mediated immune responses, supporting advancements in immunological research and therapeutic development.

  • Mouse IFN-gamma ELISpot Kit
  • Human IFN-gamma ELISpot Kit
  • Primate IFN-gamma ELISpot Kit
  • Rat IFN-gamma ELISpot Kit
  • Mouse IL-4 ELISpot Kit
  • Mouse Granzyme B ELISpot Kit

Europe, North America, and Asia-Pacific

In March 2021, Bio-Techne announced that the company will be organizing its webinar session on arrays in cancer research. This webinar is focused on demonstrating the latest diagnostic kits and their application in cancer research, thereby anticipated to enhance the revenue for the market



Mabtech is a specialized provider of monoclonal antibodies and immunoassays, including high-performance ELISpot and FluoroSpot kits. Their products are well-regarded for their reliability and precision in detecting cytokine secretion at the single-cell level. Mabtech's assays are extensively used in immunological research, vaccine development, and monitoring of immune responses in various diseases.

  • Human IL-1α ELISpotBASIC kit (ALP)
  • Human IL-1α ELISpotBASIC kit (HRP)
  • Human IL-3 ELISpotPLUS kit (ALP)
  • Human EBI3 ELISpotPLUS kit (ALP)
  • Human EBI3 ELISpotPLUS kit (HRP)

Europe, North America, and Asia-Pacific

In April 2021, Mabtech announced on their website that the company launched their new products IFN-α (mAbs, ELISABASIC, and ELISpotBASIC) using mouse strain. They demonstrated that these kits are much better at detection than the company's available competitive kits, thereby improving their product portfolio.


Thermo Fisher Scientific Inc.

Thermo Fisher Scientific is a global leader in scientific research services and products, offering a wide array of tools for the life sciences. In the context of ELISpot and FluoroSpot assays, Thermo Fisher provides robust reagents, kits, and instruments designed to deliver high sensitivity and specificity in detecting immune responses. Their products are integral to research in immunology, aiding in the development of vaccines and immunotherapies.

  • IL-21 Mouse ELISPOT Kit
  • Human IFN gamma ELISPOT
  • eBioscience ELISA/ELISPOT Diluent (5X)
  • ELISA/ELISPOT Coating Buffer Powder

Europe, North America, and Asia-Pacific

In February 2021, Thermo Fisher Scientific Inc. announced it has won six awards in the annual CMO Leadership Awards. The awards presented by Life Science Leader and Outsourced Pharma recognize top contract manufacturing partners as evaluated by biopharma and biotech companies. It is estimated that this recognition anticipated strengthening company’s footprint in the global market and leading to an upsurge company’s growth in the coming years.


The market is supported by prominent companies such as BD, Oxford Immunotec, Bio-Techne, Mabtech, and Thermo Fisher Scientific, each offering specialized products and technologies to advance immunological research and diagnostics. BD and Thermo Fisher provide comprehensive instrumentation and reagents that enhance assay sensitivity and accuracy. Oxford Immunotec is notable for its T-SPOT technology, which is widely used in clinical diagnostics. Bio-Techne and Mabtech offer high-quality assay kits and reader systems, which are crucial for precise measurement of cytokine production. Together, these companies drive innovation and reliability in the field, facilitating critical advancements in vaccine development and immunotherapy research.

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